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Choline chloride, the most common and economical form of choline, is mainly for mixing additives to animal feed. Choline usually classified as complex vitamin B (often called vitamin B4), maintain the physiological functions of animals’ bodies as a low-molecular organic compound that can be synthesized in vivo, but usually required in feed as a single vitamin, the largest demand in feed additive. It can regulate fat metabolism and transformation in vivo, whereby preventing abnormal fat accumulation in liver and kidney and tissue-degenerating, promote re-formation of amino acids and aid the utilization of amino acids, sparing methionine partly.